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Old 09-30-2019, 02:47 PM   #3
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Location: australia

Join Date: Sep 2019
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Originally Posted by cmbetts View Post
Yes, but with levels of difficulty ranging from trivial to very difficult. If they're for an NGS related application, there's a good chance they're just common primers you can pick from a catalogue or find the sequence by google. You could also potentially track down sequences if they've been used in a published paper.
If you've been using them for sequencing, they're probably in your FASTQ somewhere with the main issue being that it can be hard to derived their length, but can be guessed at by Tm analysis.
If all else fails, you can clone and sequence them, or make a ssDNA NGS library and sequence them if it's a big multiplex pool.

Thanks for that. How would I get an idea of how much it would cost to sequence some unknown primers?
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