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Old 10-24-2019, 10:29 AM   #6
Location: Bay area

Join Date: Jun 2012
Posts: 77

I don't understand how you could have gotten to that point...? But anyway...

If you still have the amplicon, and some residual primer left over, then send them out for Sanger sequencing to identify the putative primer sites. Adjust length according to desired Tm and then get them synthesized (it is always cheaper to synthesize).

You could do this within a week.
austinso is offline   Reply With Quote