Hello to everyone from a new user -I am trying to learn Casava 1.8.2. I am working with a single read 50 bp read and 7bp index run(6 base index--some samples are TruSeq RNA and some lanes have small RNA indices). After running the configureBcltoFastq script I ran make (assuming it does read 1 and read2 for index?). It sttarts but then stops at one of teh lanes giving an error that it "Failed to parse the options:
*** sample-dir not valid: number of directories must match the number of barcodes ***" I did notice that it is reporting all 6 multiplex barcodes and an additional one which it calls undetermined.
Any input? Thanks much!
*** sample-dir not valid: number of directories must match the number of barcodes ***" I did notice that it is reporting all 6 multiplex barcodes and an additional one which it calls undetermined.
Any input? Thanks much!
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