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Old 07-15-2013, 04:22 AM   #2
Location: Rockville, MD

Join Date: Apr 2011
Posts: 23

Probably not. You'll get about 5M reads on a good day with the Ion318 - which is good enough for polyA+ transcriptome sequencing; comparable to what you might expect from an Affymetrix array. We also do a lot of small RNA sequencing on the PGM - and it (316/318) seems to work quite well, but For total Transcriptome you are going to be better off with an Ion Proton or a MiSeq. You simply need more reads for that type of sample.
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