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Old 07-01-2018, 06:46 AM   #14
fgilpara
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Location: Barcelona

Join Date: Oct 2017
Posts: 10
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nucacidhunter, thankyou, you were right, the sequence lacked that nucleotide.
Im trying to send you a private message but its not working:

Thank you, you were right. Even illumina support was telling us that the original sequence was ok but some experiments worked better with that nucleotide addition, but that they cant help us further cause its a custom primers experiment. Your solution worked, we adquired primers with the lacking nucleotide and it worked like charm. We suspect that the mismatch in the another amplicon was not sufficient to make it fail but changed that primers too. Thanks!!!!!

Last edited by fgilpara; 07-01-2018 at 06:51 AM.
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