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Old 02-21-2012, 05:37 AM   #24
joss211
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Location: boston

Join Date: Mar 2011
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It seems to me like the acceptability of a 4% error rate would depend on the sample type. One advantage of sequencing clusters (or beads) is that each read is a pretty accurate determination of sequence derived from a single template. I am a bit of a statistics moron, but it seems like if your starting material is impure (e.g. a tumor sample), that it would be easier to distinguish normal sequence from minority-contributor sequence (say 5%) if you are 99.9% sure of each base in a read than if you are 96% sure of each base in a read. While 200x coverage might be sufficient for the former, would it be sufficient for the latter?
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