Each MiSeq sequencing kit is for one run only and you can multiplex several libraries in one run depending on number of required reads. Multiplexing can be tailored to obtain equal or unequal number of reads for each library.
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Originally posted by GA-J View PostDoes anybody know the different between NextSeq High output 300cycles and 150cycles kit? Only the reagent volume different? May I combine 2 of 150cycles kit for a 300 cycles run?
[Disclaimer: My answer is not based on first hand knowledge but on assuming that the NextSeq operates like the MiSeq in this respect.]
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