SEQanswers - View Single Post - Is it ok to combine RR run and HO run data for downstream data analysis?

weigrc · 05-03-2015, 05:30 PM

This project is for Reduced Representation Bisulfite Sequencing (RRBS).

I was thinking it should be no problem with combining RR PE50 and the HO PE50 (derived from HO PE100) data. But Illumina tech support told that "...though both HO and RR running on HiSeq but it shares different chemistry. If you just want to combine the data set and not care the downstream analysis, I think it should be fine...", which makes me hesitate doing this. Anyway, I'd do another run of RR PE50. Thanks for your suggestions.

Regards,
Wei

05-03-2015, 05:30 PM	#5
weigrc Member Location: Hong Kong Join Date: Oct 2011 Posts: 46	This project is for Reduced Representation Bisulfite Sequencing (RRBS). I was thinking it should be no problem with combining RR PE50 and the HO PE50 (derived from HO PE100) data. But Illumina tech support told that "...though both HO and RR running on HiSeq but it shares different chemistry. If you just want to combine the data set and not care the downstream analysis, I think it should be fine...", which makes me hesitate doing this. Anyway, I'd do another run of RR PE50. Thanks for your suggestions. Regards, Wei