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Old 03-02-2019, 05:43 PM   #5
ksqb
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Location: Bethesda

Join Date: Oct 2018
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Thank you, AndrewP. The samples are mammalian cells in culture and I am planning to isolate the RNA using RNAeasy. A Thermo Fisher workflow suggests that cells are washed by pelleting to get rid of the RNAlater and then processed for RNA isolation. I heard that RNAlater causes some cell lysis so may cause loss of RNA. I am in a bit of a quandary as to how to process with RNA extraction without losing sample.
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