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Old 03-04-2019, 06:14 AM   #7
Marc_Jones
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Location: UK

Join Date: Jun 2017
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I've snap froze tissues without any loss of RNA integrity. The biggest effect on RNA integrity is the time taken from sacrifice to freezing the tissue.

The main reason we froze was because wanted to section, stain and macro-dissect regions for sequencing. FYI RNAlater does funky things to cutting media (OCT).
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