Hi all,
I am having some issues with my Ampure cleanup step and would love some troubleshooting help.
I'd say that every time I use the ampure beads (at 1.8 ratio) I lose about 50% of my product, which seems like A LOT. (I've tried my best to optimize the pcr conditions, but I think the biggest problem is that my template DNA is super crappy (literally - it is extracted from feces) and full of inhibitors.)
I'm already struggling to get enough ng of product made for NGS, so I'm pretty tired of losing it (and this high quality taq is expensive!) The beads expire in 2013. I make new EtOH every time. Might anyone have any idea about what could be going wrong??
I am having some issues with my Ampure cleanup step and would love some troubleshooting help.
I'd say that every time I use the ampure beads (at 1.8 ratio) I lose about 50% of my product, which seems like A LOT. (I've tried my best to optimize the pcr conditions, but I think the biggest problem is that my template DNA is super crappy (literally - it is extracted from feces) and full of inhibitors.)
I'm already struggling to get enough ng of product made for NGS, so I'm pretty tired of losing it (and this high quality taq is expensive!) The beads expire in 2013. I make new EtOH every time. Might anyone have any idea about what could be going wrong??
Comment