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Old 08-22-2014, 05:05 AM   #15
ehdezsanabria
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Location: belgium

Join Date: Aug 2014
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Quote:
Originally Posted by chadn737 View Post
I have used Turbo DNase followed by cleanup on Qiagen columns. The Turbo DNase kit does provide an inactivation agent that is supposed to remove the enzyme, but time and again I find that the inactivation agent fails to adequately remove the enzyme (I have been using it for ~ 5 years now). For more routine uses, I'll use the inactivation agent, but for my more valuable samples, I always follow up with cleanup on a column.
Hi chadn 737,
We did the DNA removal using the Turbo DNase from Ambionbut used phenol:chloroform to inactivate the enzyme. Do you think this step is necessary or else, can you proceed directly to using the RNA clean-up using a kit (Qiagen)?
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