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Old 11-22-2016, 10:16 PM   #18
Amar
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Location: Australia

Join Date: Nov 2016
Posts: 2
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That's really interesting thanks DStephens@NuGEN. I never knew the RNAeasy columns can be used for clean up.

MatthewHaas: The turbo DNase kit is fantastic and in my experience the inactivation buffer works great. Just be sure to stay well away from the inactivation pellet. I have also been told that DNAse is extremely fragile and sensitive to heat. cDNA synthesis includes a heat-inactivation step, which should hopefully degrade DNAse. But as DStephens@NuGEN said, if the samples are precious then perform a cleanup step.

Good luck!
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