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Old 09-18-2012, 08:04 AM   #2
Location: Beltsville, MD, USA

Join Date: Jul 2012
Posts: 24

In my experience on-column DNase treatment is never as efficient at removing gDNA as in-solution DNase treatment. Anytime an RNA extraction protocol has an on-column DNase treatment step, I skip it, and follow up the extraction with Turbo DNase. With the Turbo kit you don't have to worry about purifying your RNA afterwards as the final step of the protocol uses an inactivation reagent to remove the enzyme and divalent cations from the RNA.
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