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Old 09-18-2012, 03:59 PM   #4
Location: Beltsville, MD, USA

Join Date: Jul 2012
Posts: 24

Happy to help.

I prefer to use vacuum centrifugation to concentrate my RNA, however, when doing so try to avoid spinning the RNA under vacuum for prolonged periods of time. I also typically never concentrate a volume greater than 40uL as I've had issues with RNA loss and degradation with larger volumes. Aliquot your sample into multiple fractions if you have to--this cuts the spin time down as well.

With my equipment, it takes about 10-12 minutes to concentrate a 40uL volume down to about 15-20uL.
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