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Old 09-19-2012, 03:30 PM   #6
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Location: New Zealand

Join Date: Sep 2012
Posts: 6

Thanks for that! Further to this, once I have obtained the ideal total RNA concentration, (200 ng/ul) I am going to try and sequence all of the small RNA (ie microRNA and piwiRNA) using the Illumina TruSeq small RNA library preparation and then sequence on the HiSeq Illumina. Is this feasible or is it better to focus on one family of small RNA?
Thank you
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