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Old 10-01-2012, 07:03 AM   #10
Johan de Bondt
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Location: Netherlands

Join Date: Oct 2012
Posts: 1
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Quote:
Originally Posted by chadn737 View Post
I have used Turbo DNase followed by cleanup on Qiagen columns. The Turbo DNase kit does provide an inactivation agent that is supposed to remove the enzyme, but time and again I find that the inactivation agent fails to adequately remove the enzyme (I have been using it for ~ 5 years now). For more routine uses, I'll use the inactivation agent, but for my more valuable samples, I always follow up with cleanup on a column.
If someone would be interested, we have developed an IP protected magnetic bead with active DNase on the surface. Perhaps an elegant manner of removing the DNA without loosing sample or diluting it! If anyone would be interested in this feel free to send me a mail.
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