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Old 03-29-2017, 12:12 PM   #4
Location: Illinois

Join Date: Oct 2014
Posts: 37

We haven't dealt with this specific situation before, but with that length of identical sequence I would probably aim a little higher with the PhiX for the first attempt (20-30%). Would be interested to hear if other folks have sequenced this kind of library on the NextSeq. Once you convince yourself you can get good data from the high PhiX spike-in, you can titrate it down on future runs so you don't waste so many reads.
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