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Old 09-03-2017, 12:58 PM   #7
dlin82
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Location: Vancouver

Join Date: May 2017
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Hi - sorry to revive an old thread, we are gearing up our own very first RRBS run on the NextSeq500 so wanted to check in if 30% is actually a good amount of PhiX to spike in. Are the quality of clustering consistent?

Consulting with Illumina and other sources they suggest a starting amount of 40-50% (more like 50%) PhiX, and you really do lose quite a lot of reads with that amount so I would like to avoid that at all if possible. Thanks so much everyone!
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