Has anyone experienced their MiSeq run just failing with no warning?
We started a run on our MiSeq, everything looked good as far as cluster density, Q scores, etc. then in the middle of the night at cycle 169 the machine essentially turned off. When I looked at the machine the next morning the computer was not responding. When the machine was turned back on and I went to the Illumina interface it just went to the main home screen page as if you were about to start a run. When I hit sequence the machine told me I forgot to do a post-run wash and acted like it had completed the run (no error messages were generated). I called Illumina and their first thought was that this was due to a glitch on the Microsoft side. After some of their reps looked through all our files they couldn't find any reason why the run would fail on the Illumina side of things. However when looking at the Microsoft side there were several error messages generated.
We are having a tech come out and look at our machine this week, but I just wanted to see if anyone else had experienced their MiSeq run failing due to glitches in the Microsoft software? Illumina didn't portray that this has been an issue in other MiSeq machines but I just want to get an idea if anyone else has had a similar issue.
Thanks for the help!
We started a run on our MiSeq, everything looked good as far as cluster density, Q scores, etc. then in the middle of the night at cycle 169 the machine essentially turned off. When I looked at the machine the next morning the computer was not responding. When the machine was turned back on and I went to the Illumina interface it just went to the main home screen page as if you were about to start a run. When I hit sequence the machine told me I forgot to do a post-run wash and acted like it had completed the run (no error messages were generated). I called Illumina and their first thought was that this was due to a glitch on the Microsoft side. After some of their reps looked through all our files they couldn't find any reason why the run would fail on the Illumina side of things. However when looking at the Microsoft side there were several error messages generated.
We are having a tech come out and look at our machine this week, but I just wanted to see if anyone else had experienced their MiSeq run failing due to glitches in the Microsoft software? Illumina didn't portray that this has been an issue in other MiSeq machines but I just want to get an idea if anyone else has had a similar issue.
Thanks for the help!
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