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Old 04-06-2016, 07:31 AM   #207
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Location: NY

Join Date: Feb 2009
Posts: 161

Originally Posted by SamCurt View Post
Thank you for the quick reply, Alex.

I also have another problem here. My new institution only has 2.4.0j on their cluster, and it'd take about a week to get a newer version installed. Do you think it's safe to run the first pass using 2.4.0j, and use its files for --sjdbFileChrStartEnd when I get, say, 2.5.1b?

Hi Sam,

this would be generally safe, however, when you publish your method, the reviewers and readers will have a bone to pick with you.
STAR does not really require installation, you can download a pre-compiled executable and run it instead of the one "installed" on your cluster.
I recommend re-generating the genome indexes for the 2.5.1b.

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