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Old 10-22-2008, 07:05 AM   #2
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Location: SF Bay Area, CA, USA

Join Date: Oct 2007
Posts: 1,358

The nanodrop number is subject to inflation based on other molecules in your sample (salts, free nucleotides, short oligos) (like any spectrophotometer).

How are you cleaning up your sample, and what is the concentration range you're in?

Overall, I have found the bioanalyzer much more accurate for quantitation prior to ePCR.
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