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Old 08-09-2013, 05:53 AM   #3
rhinoceros
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Location: sub-surface moon base

Join Date: Apr 2013
Posts: 372
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Quote:
Originally Posted by NGS group View Post
No one can give me any suggestion? It seems that such kind of questions always have no answer in this forum.
It seems to me like a simple literature search (or even google) would answer your question, but perhaps I'm wrong..

To be honest, I don't see how your method is going to work. I mean, as far as I can tell, you're extracting all the nucleotides that differ for all samples vs. reference, so you end up with files that differ in nucleotide counts. How are you going to get anything meaningful out of those files? Or are you recording also the loci of all the snips? If yes, then why not just extract those loci also from your reference?

p.s. Almost all of my questions here have gone unanswered, but I take it as a compliment

Last edited by rhinoceros; 08-09-2013 at 06:09 AM.
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