View Single Post
Old 05-08-2015, 11:37 AM   #10
DNATECH
Member
 
Location: Davis, CA

Join Date: Mar 2015
Posts: 29
Default

Hi GenoMax,

perhaps we are just being careful at the moment - since Illumina seems to be very careful and there is very little information so far. The customer samples (n=11) have been looking great so far except one; this sample had some larger low complexity component to it (which we were not aware off). For this sample the Q30 rates dropped after the first 60 to 70 bases of low complexity bases from 95% to 70%.

Quote:
Originally Posted by GenoMax View Post
@DNATECH: Based on this (and your other post) it sounds like you need "near perfect libraries" to get good data from patterned flowcells. This could be a problem for core facilities, where "variable" quality libraries come in from customers.

It would be interesting to hear about your experiences as real world customer libraries start flowing through.
DNATECH is offline   Reply With Quote