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Old 05-08-2015, 01:11 PM   #13
DNATECH
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Location: Davis, CA

Join Date: Mar 2015
Posts: 29
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Hi Pmiguel,

the basic procedure looks like:
- 5 ul of library (2 nM to 3 nM including PhiX)
- add 5 ul 0,1 N NaOH
- add 5 ul Tris (200mM)
- add 35 ul Enzyme Master Mix
- load all 50 ul onto cBot

Quote:
Originally Posted by pmiguel View Post
Wow, 2000 pM? I think the highest we ever went on the HiSeq2500 was 23 pM.

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Phillip
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