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Old 02-08-2016, 07:25 AM   #2
MU Core
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Location: Columbia, Missouri

Join Date: Apr 2008
Posts: 57
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Ghada, you're observing the presence of "dye blobs" which is the result of poor removal of unincorporated dye terminators from the post-sequencing reaction. A common issue in dye terminator sequencing. You'll want to revisit your reaction clean-up method to see if changes can be made to optimize the method. Or, you may want to consider a different approach to reaction clean-up. Performing a google search with "removing dye blobs" will provide you with information on the topic.
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