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Old 06-30-2016, 11:30 AM   #3
Senior Member
Location: New England

Join Date: Jun 2012
Posts: 200

I don't get the same results from the BR and HS assays either. I think the HS assay is just not correct at the top end of the standard curve. I usually pool samples based on the HS assay and then use qPCR to quantify the pooled library - my pooled library is often twice as concentrated as it should be based on the Qubit.

Was the quantification of the pooled 18s library very different from the 16s library or were they both in the middle of the standard curve? And you are converting the values to nM using the size (in bp) of the library?
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