Thread: 16S triplicates
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Old 10-11-2016, 09:27 AM   #4
Location: United States

Join Date: May 2014
Posts: 40

I think this may be one of the papers you're seeking out: Sequence Depth, Not PCR Replication, Improves Ecological Inference from Next Generation DNA Sequencing

The authors assess the relationship between pooled PCR replicates and diversity metrics for 16S profiling on both the 454 and the MiSeq and show little benefit to replicate PCR.
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