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Old 05-04-2018, 10:36 AM   #10
Junior Member
Location: Acela Corridor

Join Date: Dec 2017
Posts: 3

Hey Chuanwu and MU Core,

We've started to include KAPA standard 0 (from their illumina quantitation kit) to our qPCR assays and have adjusted the qPCR concentrations of our pools accordingly. In doing so we've had much more consistent cluster densities on our MiSeq/HiSeq runs.
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