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Old 05-04-2018, 01:56 PM   #11
chuanwu
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Location: USA

Join Date: May 2018
Posts: 8
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Hi DPVT,
Thank you for your reply. How did you do 10,000 or 20,000 times dilution of your samples? Did you try two consecutive 1/100 dilutions or more than 2 consecutive dilutions? Such as 50X, 200X, 2000X, 10000X etc?

By the way, have you ever measured your pooled library again by qPCR, whether it really matches?

Thanks
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