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Old 05-13-2012, 11:53 AM   #3
scbaker
Shawn Baker
 
Location: San Diego

Join Date: Aug 2008
Posts: 84
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Quote:
Originally Posted by mkdir View Post
Hello, I am new to illumina sequencing. I am wondering how many total bases I can get if I spend ~ $3000 for PE 100 bp, one lane. And how many bases can be used after quality filtering. Are there any good illumina core facilities to recommend? Thanks a lot.
$3000 for a single lane of a HiSeq2000 run seems a little expensive to me (although if you're really only running a single sample like that, a service provider/core lab might have to charge more on a per lane basis). If you put your project up on BlueSEQ's exchange, several service providers will bid on it and you might end up getting a better price.

Good luck!
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