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Old 04-24-2013, 04:06 AM   #4
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Originally Posted by illuminaGA View Post
Thank you so much.

How to improve the %PF? Do you have some advises?
This would be a good case where you should contact Illumina tech support so you can get comprehensive help from them.

As I had said before one obvious thing to try would be to load less DNA next time (you did not say what your original cluster counts were). If overloading was not the problem (if the total cluster counts were within limits) then perhaps something else has gone wrong with the tag read/libraries.

You should contact Illumina support (or your local FAS) since your original post seems to indicate that this has been a repeat problem.
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