Hello everybody,
I am really new to this field and trying to learn to use Galaxy.
Just wondering if there is any criteria used to determine the "right" quality of data and at what threshold do you discard or trim your sequences? In what situation will you decide to trim?
I used the FastQC in galaxy
will you trim the first 10 bases for the per base content below? GC content, Kmer content has fluctuation for the first 10 bases.
any advice?
I am really new to this field and trying to learn to use Galaxy.
Just wondering if there is any criteria used to determine the "right" quality of data and at what threshold do you discard or trim your sequences? In what situation will you decide to trim?
I used the FastQC in galaxy
will you trim the first 10 bases for the per base content below? GC content, Kmer content has fluctuation for the first 10 bases.
any advice?
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