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Old 11-25-2013, 01:44 PM   #15
Location: Australia

Join Date: Nov 2013
Posts: 16

Hi Evelien,

The combination was quite straight forward. My input DNA was from plant leaf tissue freshly extracted using a Qiagen DNeasy kit (final elution in DNase/RNase free ultra pure water). Then simply follow the manuals as-is. No specific fragmentation is needed as the bisulphite treatment does this anyway. The range of fragments post treatment was between 150 and 2000 bp (peak around 300 bp) measured on a bioanalyzer high sensitivity chip.

Hope that helps,

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