Thread: No usable reads
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Old 05-21-2018, 02:11 PM   #4
Location: Brazil

Join Date: Aug 2015
Posts: 92

Ciao Isabella!
I see that the consensus key is very low... I would think that you had an issue with either the polymerase or the sequencing primer... or with the run itself.
You do have a huge % of "no template" ISPs and very low policlonality. You mention the dilution factor of your libraries, do you suspect having used too few DNA at the emulsion PCR step? But then you should have likely removed the empty beads at the ES purification step, having very few ISP left, and you'd see a lot of test fragment ISPs in the run. (Do you by chance save those 2ul of ISPs for the Ion Sphere Quality Control Kit on the Qubit?)
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