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Old 08-14-2013, 07:26 AM   #3
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Location: Europe

Join Date: Aug 2013
Posts: 2

Originally Posted by dpryan View Post
Did you run fastQC (or any other QC software) on these reads?
Yes I ran FastQC. The Per base sequence quality is in the green zone for all of them. In some the right reads are slightly lower than the left, but not much.

The only other thing I notice with FastQC is that 'Sequence Duplication Levels' are very high (>=55.7%) . I don't know whether this could influence mapping though.

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