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Old 03-07-2014, 05:09 AM   #5
cement_head
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Location: Oxford, Ohio

Join Date: Mar 2012
Posts: 250
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Quote:
Originally Posted by Zaag View Post
I will be trying this in a week or two, so, did you make any progress? ;-)

From what I understand it should be possible to remove the primers from ampliseq and subsequently ligate adapters, so I was planning on ligating MiSeq adapters instead of PGM adapters.
I wouldn't do ligation, just do another round of PCR with primers for Illumina flow cells (get them from IDT).
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