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Old 06-16-2014, 12:56 AM   #5
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232

N.B. some kits reverse things, such that read #1 will be on the - strand if the mRNA is on the + strand. I think those are mostly for SOLiD sequencing, so you're unlikely to bump into them anymore.
Some kits like TruSeq Stranded RNA-Seq Sample Prep Kit from Illumina results in read #1 on the opposite strand of mRNA as illustrated in the attachment.
Attached Files
File Type: pdf Illumina stranded RNAseq mapping.pdf (158.6 KB, 224 views)

Last edited by nucacidhunter; 06-16-2014 at 12:59 AM.
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