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Old 12-02-2016, 03:48 AM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232
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Most likely reason for overclustering in this case would be inaccurate library quantification and high clustering of low diversity library will result in low quality. If reducing loading from 15 pM to 10 pM reduced cluster number then you might review the quantification.
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