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Old 01-29-2018, 02:39 PM   #2
jwfoley
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Location: Stanford

Join Date: Jun 2009
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Libraries over 2 kb don't tend to work on Illumina sequencers, so do you really care what the concentration of those molecules is?

Also, are you doing a TaqMan qPCR or SYBR? If it's SYBR, you have to factor in the average molecule size, so you're still dependent on the Bioanalyzer anyway.
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