View Single Post
Old 07-30-2018, 04:43 PM   #2
Senior Member
Location: Bethesda MD

Join Date: Oct 2009
Posts: 505

Yes, adapter dimers can anneal to insert-containing molecules via the adapter ends. The simplest solution is to perform one new round of PCR using ~1/2 (or less, depending upon the amount) of the library as template, followed by clean-up and QC. Insert-containing library and adapter dimer will now migrate at their true sizes.
HESmith is offline   Reply With Quote