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Old 07-30-2018, 04:43 PM   #2
HESmith
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Location: Bethesda MD

Join Date: Oct 2009
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Yes, adapter dimers can anneal to insert-containing molecules via the adapter ends. The simplest solution is to perform one new round of PCR using ~1/2 (or less, depending upon the amount) of the library as template, followed by clean-up and QC. Insert-containing library and adapter dimer will now migrate at their true sizes.
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