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Old 03-05-2011, 12:43 AM   #8
BaCh
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Location: Germany

Join Date: May 2008
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Quote:
Originally Posted by sklages View Post
I had a 3mio-titanium-reads-human-non-normalzied-cDNA dataset;one fourth of the whole library representing the same gene ... Newbler even failed to map and assemble this set (crashed during consensus calculation).
*cough* 750k times the same gene? I would not expect any program to really assemble that de-novo if not specially primed. In mapping too, the coverage might be somewhat on the unexpected side.

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