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Old 04-08-2011, 07:30 AM   #4
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Location: St. Louis

Join Date: Dec 2010
Posts: 535

If we can start with 3ug DNA we can get away with 7 or fewer PCR cycles. The protocol states to do 4-6 but when I've done 8 cycles I haven't had any issues. 16 prior to hybridization seems quite high, though. I communicated with an Agilent rep who told me that duplicates are mainly caused by too many cycles pre-hyb.
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