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Old 04-29-2011, 05:52 AM   #5
Location: Germany

Join Date: Mar 2011
Posts: 68

I think the number of reads and the target region size is important as well.

But nevertheless we have the duplication problem as well. We used the Agilent AllExon-Kit and sequenced with the HiSeq (one lane per sample). We observed a duplication rate of 40-50%, although we sticked to the protocol. We observe, that the duplication rate with the GA II is much lower than with the HiSeq. Does anyone observe the same?

How many reads did you map to which region size and which duplication rate did you observe?

@Heisman: Did you sequence with the HiSeq or the GA II? How many reads did you receive?
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