I was just wondering what are the ligation efficiencies on illumina libraries for people working on it.I get poor efficiencies on QPCR, ranging from 40-5%, and even worst for no PCR libraries.I calculate my efficiency as mentioned below.
QPCR/Bioanalyzer Conc.*100
I did compare on Taqman as wel as sybrgreen kapa reagents, but din't really had a significant difference.
Please let me know if anybody is having similar problems
QPCR/Bioanalyzer Conc.*100
I did compare on Taqman as wel as sybrgreen kapa reagents, but din't really had a significant difference.
Please let me know if anybody is having similar problems
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