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Old 10-24-2012, 05:02 PM   #3
fabrice
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Location: paris

Join Date: Oct 2009
Posts: 86
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swbarnes2,

Thank you for your clarify. Yes. I did not do a strand-specific library prepare. But I still have bit of problem to understand why I should expect to see reads in both directions.
The mRNA is only in the one direction, when sequence we should also see one direction.
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