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Old 10-27-2011, 06:15 PM   #7
skblazer
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Location: Massachusetts

Join Date: Feb 2009
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Thanks Phillip. The reaction mix is super expensive, thus I think I still need this PCR purification.
Quote:
Originally Posted by pmiguel View Post
In principle, the amounts of primers and other small stuff in a PCR reaction might overwhelm the capacity of some gels. Some tiny amount of small material might end up co-migrating with the DNA you intend to cut out.

More likely, the PCR reactants will slightly alter the mobility of your DNA, making it not run at the same rate as your ladder. So your size estimate may be off. I guess you could add 10x PCR reaction mix (with polymerase!) to your ladder to give it the same buffer and ionic strength as your PCR reaction...

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Phillip
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