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Old 02-20-2013, 11:40 AM   #1
Location: Carrollton, GA

Join Date: May 2011
Posts: 27
Default GOSSAMER hardware recommendation?

I will need to assemble a genome from two PE250 MiSeq runs generating a total of about 12 to 14 Gbp of sequence. Based on some preliminary alignments to genes I had previously cloned and sequenced this would, on average, be better than 10x coverage. Since I don't have access to or funds for time on a computer with sufficient RAM to run something like Velvet, I was looking at Gossamer as an assembler with low hardware resource requirements. My question is this. I may be able to configure a VM for a short amount of time and I am wondering what would be a reasonable amount of vRAM and minimum number of CPU cores to get the assembly done in a reasonable time period.
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