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Old 06-20-2012, 01:24 AM   #3
TonyBrooks
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Location: London

Join Date: Jun 2009
Posts: 298
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We used a TruSeq RNA kit that was 9 months passed use-by date and it still produced excellent libraries. We followed SS00's advice and aliquoted the reactions out into smaller volumes when the kit arrived and stored everything at the recommended temperature (written on each individual tube).
We don't use Ampure beads that are out of date because we've found they don't keep so well.
There are also alternative prep kits that are supplied in smaller pack sizes which may be more suitable if you don't want the hassle of aliquoting everything out (Bioo, NEB). Personally, I'm not so keen on the TruSeq DNA kits as size selection is a real problem. The TruSeq RNA kits are excellent though.
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