Hi everyone,
I just got my first PacBio data in and would like some advice on how to handle the data. I submitted a 1KB amplicon library of ~ 5K variants, and requested that the library be run on CCS mode (90 min movie). Instead, the sequencing facility ran the library as a 240 min movie (P6 C2 chemistry). For this run, the P1 productivity was 56% and P2 productivity was 37%. The quality of "Reads of Insert" was 0.96. I was planning to use the Long Amplicon Analysis in the SMRT Portal when I get the CCS data, but now I am a bit unsure about how to work with the data.
Can my data (from 240 min movie) be treated the same way as a CCS run for data analysis ? Or does a CCS run and subsequent analysis treat the data differently compared to assembling a consensus from the sub-reads of a traditional run ?
Many thanks from a PacBio newbie !
I just got my first PacBio data in and would like some advice on how to handle the data. I submitted a 1KB amplicon library of ~ 5K variants, and requested that the library be run on CCS mode (90 min movie). Instead, the sequencing facility ran the library as a 240 min movie (P6 C2 chemistry). For this run, the P1 productivity was 56% and P2 productivity was 37%. The quality of "Reads of Insert" was 0.96. I was planning to use the Long Amplicon Analysis in the SMRT Portal when I get the CCS data, but now I am a bit unsure about how to work with the data.
Can my data (from 240 min movie) be treated the same way as a CCS run for data analysis ? Or does a CCS run and subsequent analysis treat the data differently compared to assembling a consensus from the sub-reads of a traditional run ?
Many thanks from a PacBio newbie !
Comment